Molecular Cloning of cDNAs Encoding Vitamin D3 25-Hydroxylase and 25-Hydroxyvitamin D3 24-Hydroxylase
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چکیده
منابع مشابه
25-Hydroxyvitamin D3-24-hydroxylase in rat kidney mitochondria.
Assay conditions for the measurement of 25-hydroxyvitamin D3-24-hydroxylase activity in rat kidney mitochondria have been worked out. The product, 24,25-dihydroxyvitamin D3 was quantitated either by high pressure liquid chromatography or by isotope dilution-mass spectrometry. By these procedures, the enzyme activity could be measured with saturating concentration (greater than 2.5 X 10(-6) M) o...
متن کاملLocalization of 25-hydroxyvitamin D3 1 alpha-hydroxylase and 24-hydroxylase along the rat nephron.
Defined nephron segments were microdissected from the kidney of vitamin D-deficient rats, normal rats, and normal rats treated with 1 alpha, 25-dihydroxyvitamin D3 [1 alpha, 25-(OH)2D3]. Tubule segments were incubated with 3H]labeled 25-hydroxyvitamin D3 and the rates of production of 3H]labeled 1 alpha, 25-(OH)2D3 and 24,25-dihydroxyvitamin D3 [24,25-(OH)2D3] were determined. Nephron segments ...
متن کاملExtrarenal Expression of 25-Hydroxyvitamin D3-1a-Hydroxylase*
The mitochondrial enzyme 25-hydroxyvitamin D3-1a-hydroxylase (1a-hydroxylase) plays an important role in calcium homeostasis by catalyzing synthesis of the active form of vitamin D, 1,25-dihydroxyvitamin D3, in the kidney. However, enzyme activity assays indicate that 1a-hydroxylase is also expressed in a variety of extrarenal tissues; recent cloning of cDNAs for 1a-hydroxylase in different spe...
متن کاملSubcellular localization of vitamin D3 25-hydroxylase in human liver.
Vitamin D3 25-hydroxylase activity was measured in subcellular and submitochondrial fractions of human liver. Quantitation of 25-hydroxyvitamin D3 was based on high performance liquid chromatography. Vitamin D3 25-hydroxylase activity was detected in the mitochondrial fraction only. The mitochondrial 25-hydroxylase activity was linear with time up to 60 min and with mitochondrial protein up to ...
متن کاملCloning and expression of rat 25-hydroxyvitamin D3-1alpha-hydroxylase cDNA.
A full-length cDNA for the rat kidney mitochondrial cytochrome P450 mixed function oxidase, 25-hydroxyvitamin D3-1alpha-hydroxylase (P4501alpha), was cloned from a vitamin D-deficient rat kidney cDNA library and subcloned into the mammalian expression vector pcDNA 3.1(+). When P4501alpha cDNA was transfected into COS-7 transformed monkey kidney cells, they expressed 25-hydroxyvitamin D3-1alpha-...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1992
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.38.special_100