Molecular Characterizations of Cytolethal Distending Toxin Produced by Providencia alcalifaciens Strains Isolated from Patients with Diarrhea
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چکیده
منابع مشابه
Molecular characterizations of cytolethal distending toxin produced by Providencia alcalifaciens strains isolated from patients with diarrhea.
Cytolethal distending toxins (CDTs), which block eukaryotic cell proliferation by acting as inhibitory cyclomodulins, are produced by diverse groups of Gram-negative bacteria. Active CDT is composed of three polypeptides--CdtA, CdtB, and CdtC--encoded by the genes cdtA, cdtB, and cdtC, respectively. We developed a PCR-restriction fragment length polymorphism assay for the detection and differen...
متن کاملAssociation of cytolethal distending toxin locus cdtB with enteropathogenic Escherichia coli isolated from patients with acute diarrhea in Calcutta, India.
Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These isolates were identified as being enteropathogenic E. coli (EPEC). In a retrospective study using a probe hybridization assay, 6 of 138 EPEC strains were found to harbor the cdtB locus. cdtB-positive isol...
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Providencia alcalifaciens is a member of the family Enterobacteriaceae. There are reports that P. alcalifaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is known. We studied P. alcalifaciens isolated from a child and two adults with diarrhea for enteropathogenicity. The three isolates did not exhibit any characteristic adherence to cultured HEp-2 cell monolayers, and...
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The aim of this study was to investigate the prevalence of virulence and Cytolethal Distending Toxin (CDT) genes in the Campylobacter isolates from intestinal contents and gall bladders of broilers and, to evalute their cytotoxic effects on HeLa cell cultures. These genes play important roles in bacterial adherence to intestinal mucosa, flagella-mediated motility, invasive capability and the ab...
متن کاملPathogenesis of Providencia alcalifaciens-Induced Diarrhea
Providencia akalifaciens is a member of the family Enterobacteriaceae. There are reports that P. akalfaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is not known. We studied P. akalfaciens isolated from a child and two adults with diarrhea for enteropathogenicity. The three isolates did not exhibit any characteristic adherence to cultured HEp-2 cell monolayers, and ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2012
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.05831-11