MOLECULAR CHARACTERIZATION SOME BACTERIAL PATHOGENS CAUSING BOVINE MASTITIS WITH SPECIAL REFERENCE TO Mycoplasma bovis

In dairy industry, bovine mastitis is the most prevalent disease, which reduces milk production and causes economic losses. This study was conducted to estimate prevalence of Mycoplasma bovis some bacteria causing in farms partial sequencing 16SrRNA target genes Quinolones Resistance Determining Regions (QRDRs) (gyrA parC) M. isolates. 370 samples were obtained from located villages Fayoum governorate, Egypt. The examined (8,91%) positive for California test (CMT). Multiplex RT-PCR used recognition microorganisms (Staphylococcus (S.) aureus, Streptococcus species (spp.), Escherichia (E.) coli, (M.) bovis) mastitic milk. results revealed that E. coli predominant (84.8%) followed by S. aureus (81.8%) while lowest one (51.5%). Mixed infection with two or more bacterial agents also identified. All 33 diagnosed mixed spp. (36.36%), (21.21%), rephrase bovis, (6.06%). sequence analysis illustrated a high similarity isolates strains previously deposited GenBank recovered same locality. gyrA amino acids showed no substitution but 100% worldwide. However, acid parC, at positions 2 (Gln Arg) (CAG >>CGT), 75 (Ile Ser) (ATT>>AGC), 79 (Asn Asp) (AAC>>GAT). Sequence can lead creation appropriate treatment control measures multiplex RT-PCR, be exploited as standard diagnostic method major pathogens.