Molecular characterization of bluetongue virus serotype 2

Bluetongue is an economically major infectious, arthropod vector borne, viral disease of many free ranging and captive wild animals as well domestic animals. There are serotypes circulating in the world. The current study was undertaken having objective to characterize BTV 2(M11) isolate that engaged for vaccine trial. BHK21 cell lines were utilized cultivation serotype 2 followed by its RNA extracted. Further, Reverse Transcriptase-PCR standardized detection 2by targeting segment 7 (VP7) gene. Characterization at molecular level conducted sequencing VP7 By using available sequences Genbank database, compared. Further analysis denoted that, all nucleotide sequestered into seven phylogenetic clades. homogeneity BTV-2 (M11) with Australian Chinese isolates BTV-15 Taiwan 75%, 66%, 74% respectively. signified nearly compared BTV-12 Brazil BTVTPT.