Molecular characterization and protective efficacy of vacuolar protein sorting 29 from Eimeria tenella

Introduction Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 Eimeria spp. Methods Here, we cloned prokaryotically expressed partial sequence tenella (EtVPS29) with RT-PCR engineered strain Escherichia coli respectively. The localization in E. sporozoites was investigated immunofluorescence (IFA) overexpression assays. And its protective efficacy against infection chickens animal protection test. Results An EtVPS29 gene fragment an ORF reading frame 549 bp cloned. band size recombinant protein, rEtVPS29, approximately 39 kDa recognized by chicken anti- positive serum. observed widely distributing cytoplasm IFA rEtVPS29 significantly increased average body weight gain decreased mean lesion score oocyst output chickens. relative rate rEtVPS29-immunized group 62.9%, which higher than that unimmunized challenged (P < 0.05). percentage reduced immunized 32.2%. anticoccidial index 144.2. Serum ELISA also showed immunization induced high levels specific antibodies Discussion These results suggest can induce immune response potential candidate development novel vaccines infections