Molecular Characterization and Functional Analysis of GPCR Gene Bx-srh-1 in Pinewood Nematode (Bursaphelenchus xylophilus)

Bursaphelenchus xylophilus, also known as the pine wood nematode (PWD), which causes wilt disease is one of most devastating diseases affecting forests globally. G protein-coupled receptors (GPCRs) are crucial in many biological processes and serve privileged points communication between cells surrounding environment. α-pinene has been found to play a role combating infection, colonization, early stages pathogenesis caused by B. xylophilus. In this study, we investigated molecular characteristics functions GPCR gene Bx-srh-1 Fluorescence situ hybridization (FISH) was performed determine spatial expression patterns The results indicated that expressed intestine subcutaneous tissues J2 J3 juveniles spicules adult males vulvae females. RNA interference (RNAi) used analyze function, examined xylophilus under stress. involved reproductive ability pathogenicity xylophilus; levels significantly increased after exposure for 12 h, they peaked at 48 h. Silencing may therefore lead reduction reproduction pathogenicity. These demonstrate related feeding behavior, reproduction, pathogenicity, resistance process