Molecular Characterization and Antibiogram of Staphylococcus and Pseudomonas Species Isolated from Patients Attending Rivers State Teaching Hospital, Nigeria

Disease of ear, nose and throat (ENT) affect the functioning adults as well children, often with significant challenge to daily life affected patients. Staphylococcus Pseudomonas species are most prevalent etiologic agent ENT infections. Therefore aim this study was determine antibiogram molecularly Characterized in Ear Throat among Patients Attending Rivers State Teaching Hospital. Total number eighty samples swab, aural (ear) swab were collected aseptically using sterile Evepon sticks sample properly labelled patients' number, date side. The swabs immediately transported a transportation medium cotton plugged test tube microbiology laboratory for further analysis. Qquestionnaires administered degraphic data collections. Each inoculated onto Mannitol salt agar Cetrimide plates isolation respectively. incubated at 37°C examined growth after 24-48 hours. Pure culture isolates primarily identified biochemically confirmed molecular approaches. Antibiogram performed by Kirby-Bauer disk diffusion method on Mueller-Hinton according Clinical Laboratory Standards Institute guideline. Results shows that out 50 ear 30 samples, 17 (34%) 8 (26.7%) positive spp. While (16%) 4 (13.7%) positive. results fluorescens NR_115715.1, aeruginosa NR_113599.1.
 aureus NR_113956.1. predominates isolated from both swabs. revealed commonly uses antibiotics especially Ceftazidime, Cefuroxime, Cloxacillin Gentamicin Augmentin 100% resistant only Ofloxacin, Ceftriaxone Nitrofurantoin susceptible percentage 80.2%, 85.7% 90%. findings points high level frequent indescrimate patient suffering symptoms resulting difficulty treatment infections common antibiotics. It is strongly recommended ccontinuous monitoring patients required preparing antibiograms. These susceptibility will help physician deciding empirical regime