Molecular characterisation of plasma membrane-derived vesicles

Halt Proliferation and Induce Differentiation Human Plasma Membrane-Derived Vesicles

plasma-membrane vesicles

Transport of [14C]tetraethylammonium (TEA), an organic cation, was studied in brush-border (BBMV) and basolateral (BLMV) membrane vesicles isolated from rabbit kidney cortex. In BBMV, the presence of an outwardly directed H+ gradient induced a marked stimulation of TEA uptake against its concentration gradient (overshoot phenomenon), whereas a valinomycin-induced inside-negative potential had n...

Engraftment of plasma membrane vesicles into liposomes: A new method for designing of liposome-based vaccines

Objective(s):One of the major challenges in the field of vaccine design is choosing immunogenic antigens which can induce a proper immune response against complex targets like malignant cells or recondite diseases caused by protozoan parasites such as leishmaniasis. The aim of this study was to find a way to construct artificial liposome-based cells containing fragments of target’s cell membran...

Critical fluctuations in plasma membrane vesicles.

We demonstrate critical behavior in giant plasma membrane vesicles (GPMVs) that are isolated directly from living cells. GPMVs contain two liquid phases at low temperatures and one liquid phase at high temperatures and exhibit transition temperatures in the range of 15 to 25 degrees C. In the two-phase region, line tensions linearly approach zero as temperature is increased to the transition. I...

Comparison of DABA and GABA transport into plasma membrane vesicles derived from synaptosomes.

Transport of GABA by a high-affinity transport system ( K , = M ) is thought to terminate the action of this postulated neurotransmitter. 2,4Diaminobutyric acid (DABA), a structural analogue, is taken up by neuronal elements and inhibits GABA uptake. Localization of [:1H]DABA by autoradiography has been used to identify neurons with the GABA high-affinity transport system. After reconstitution ...