Modified comet assay with Giemsa staining: a suitable method for assessing genotoxicity in rats
نویسندگان
چکیده
منابع مشابه
DNA Comet Giemsa Staining for Conventional Bright-Field Microscopy
This study was undertaken to evaluate the compatibility of Giemsa staining protocol with the comet assay. We showed, for the first time, that DNA comets can be visualized and analyzed using Giemsa staining. We generated DNA damage dose response curves for human peripheral blood lymphocytes exposed to X-ray radiation using the comet assay with either SybrGreen I or Giemsa stain. The dose respons...
متن کاملIs the Comet Assay a Sensitive Procedure for Detecting Genotoxicity?
Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine whether the power of the Comet assay to detect a low level of genotoxic potential is superior to those of other genotoxicity assays in mammali...
متن کاملModified Giemsa staining for rapid diagnosis of malaria infection.
OBJECTIVES To develop and evaluate a rapid method for the diagnosis of malaria infection by microscopy of stained blood films. SUBJECTS AND METHODS Blood specimens were collected from randomly selected confirmed malaria cases (n = 75) and suspected malaria cases (n = 175). The microscopy was done on each set of blood films stained by modified and the standard Giemsa staining methods. RESULT...
متن کاملAssessment of Genotoxicity with the Plant Comet Assay
The principle of the comet assay. During electrophoresis, damaged DNA migrates from gelembedded nuclei. Thus, nuclear DNA of individual cells acquires the appearance of a comet, with a head (undamaged DNA) and a tail (damaged DNA). The amount of DNA in the tail correlates with the number of DNA strand breaks and thus indicates the extent of DNA damage. DNA was stained with ethidium bromide and ...
متن کاملComet assay: a method to evaluate genotoxicity of nano-drug delivery system.
INTRODUCTION Drug delivery systems could induce cellular toxicity as side effect of nanomaterials. The mechanism of toxicity usually involves DNA damage. The comet assay or single cell gel electrophoresis (SCGE) is a sensitive method for detecting strand damages in the DNA of a cell with applications in genotoxicity testing and molecular epidemiology as well as fundamental research in DNA damag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
سال: 2020
ISSN: 1678-4162,0102-0935
DOI: 10.1590/1678-4162-11523