Modifications of a Candida albicans biotyping system

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Modifications of a Candida albicans biotyping system.

Methods of preparing and plating inocula onto media for the Odds and Abbott Candida albicans biotyping system (F. C. Odds and A. B. Abbott, Sabouraudia 18:301-317, 1980; Odds and Abbott, Sabouraudia 21:79-81, 1983) were altered to utilize inexpensive and commercially available supplies and equipment. The modified system correlates well with the reference system.

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UNLABELLED Candida albicans public proteomic datasets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22,000 distinct peptides at a 0.24% False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we ...

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The secreted aspartyl proteinases of Candida albicans (products of the SAP genes) are thought to contribute to virulence through their effects on Candida adherence, invasion, and pathogenicity. From a single strain of C. albicans (WO-1) which expresses a phenotypic switching system, three secreted aspartyl proteinases have been identified as determined by molecular weight and N-terminal sequenc...

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Candida albicans

Figure 1. Fluorescent protein fusions in Candida albicans. Useful fluorescent markers for tracking subcellular structures in C. albicans include: DAPI (DNA); Nop1-GFP (green fluorescent protein, localizing to the nucleolus); Hhf1-GFP (chromatin); Tub2-GFP (microtubules, green); and Tub4-mCherry fluorescent protein (spindle pole bodies, red). Micrographs courtesy of Shelly Applen and Benjamin Ha...

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[Candida albicans].

Candida albicans is an opportunistic pathogen. Its proliferation in human hosts is believed to be controlled by immunologic mechanisms. The plasma membrane of the fungus posseses an H+-ATPase (PM-ATPase) which actively extrudes protons to generate an electrochemical gradient which is used in co-transport of nutrients. This ATPase is associated with the growth, dimorphism and pathogenicity of th...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1989

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.27.6.1392-1394.1989