Modification of Pepsinogen with Pyridoxal Phosphate
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چکیده
منابع مشابه
Modification of pepsinogen with pyridoxal phosphate.
Pyridoxal-PO* binds to pepsinogen in a reaction in which the stoichiometry is highly dependent on the conformation of the protein. When pepsinogen is in its native conformation, pyridoxal-PO4 forms Schiff bases with the (r-NH2 group of Leul and the e-NH2 group of LysZS8. When the protein is mildly denatured and assumes a more extended conformation, pyridoxal-PO4 can react with the t-NH2 groups ...
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1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3. The protein fluorescence of the apo(a subform) decreased non-linearly with increase in enzyme activity and concentration of bound cofactor. 4. It is concluded that the...
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Pyridoxal 5•L-phosphate (PLP) is known to combine with bovine serum albumin to form a(1:1) complex which scarcely dissociates, even when subjected to intensive dialysis. When this complex was incubated with apo-aspartate aminotransferase (apoGOT) for an appropriate time and the preincubated mixture then submitted to the usual GOT assay, the appearance of GOT activity was obviously confirmed, in...
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Pyridoxal BP-phosphate inhibits the binding of the avian progesterone receptor to ATP-Sepharose, probably through a Schiff base interaction. It also blocks other interactions characteristic of the activated or transformed receptor, such as its binding to nuclei, DNA-cellulose, and phosphocellulose. In an attempt to explain these inhibitory effects, we have characterized the progesterone recepto...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)81263-2