Modelling an experimental systemic pseudomonas infection process

This article presents the results of an experimental model a systemic pseudomonas aeruginosa infection. can be used to develop therapeutic measures suppress infectious disease caused by antibiotic-resistant strains Pseudomonas aeruginosa. For this purpose, dry lyophilised culture (strain name: Aeruginosa No. 453, strain number 190158, obtained from Vishnevsky Institute Surgery. was pre-cultured in test tubes with meat-peptone broth (MPB), after 30 min using sterile pipette and 0.2 ml transferred agar (cetrimide agar) cultured anaerobic medium at 37±2 °C for 24 h thermostat. The is resuspended washing off surface 0.85% saline. In area, pipetted Pasteur pipette. concentration microbial cells adjusted Tarasevich turbidity standard tube. Animals are held head down so that viscera abdomen descend diaphragm. injection made lower third, left white line abdomen. site disinfected, skin fold taken needle inserted into it, turned right angles abdominal wall punctured quick thrust. blunted beforehand prevent damage intestinal loops. volume injected rabbits clinical signs infection depend on detected appropriate group. Conditions, selected allow create quickly necessary cavity laboratory animals, promoting peritonitis, criteria performance technically simple.