Modeling of bacterial keratitis of Pseudomonas aeruginosa etiology in the experimental model

Background: Pseudomonas aeruginosa keratitis demonstrates an aggressive course, high resistance to antimicrobial therapy, and it also leads a significant reduction of visual acuity. The purpose our study was develop experimental model investigate clinical features corneal lesions in rabbits. Material methods: A total 32 rabbits were divided randomly into three groups. animals the group 1 (8 rabbits) underwent scarification cornea instillation archival strain suspension P. conjunctival sac. In 2 (12 rabbits), de-epithelialization followed by microbial performed. 3 after one-day pathogen, cornea’s surface covered with sterile soft contact lens for 16–24 hours. half (6 lenses bacterial films used as modification. Results: technique I resulted conjunctivitis mild changes all cases. II, development noted animals, being defined moderate nine cases semi-severe three. III, severe purulent occurred 11 12 eyes — one. Four ulcers two abscess recorded, resulting perforations keratomalacia (2 each). Conclusions: Modeling varying severity requires complete deepithelialization segment prolonged presence pathogen on affected surface. effective way keep infectious organisms sufficient concentration is use lenses.