منابع مشابه
Preparation of Plasminogen by Affinity Chromatography
Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
متن کاملTriazine-dye affinity; chromatography.
functional resemblance of column groups to carbamoyl phosphate. The enzyme binds its two substrates, carbamoyl phosphate and L-aspartate, in an obligatory order, with carbamoyl phosphate leading (Grayson et al., 1979). If column groups are sufficiently similar to carbamoyl phosphate, the enzyme should be adsorbed more tightly in the presence of L-aspartate, owing to the ‘locking-on’ effect (O’C...
متن کاملAffinity Chromatography : A Review
Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the us...
متن کاملPurification by Affinity Chromatography
The glycine receptor of rat spinal cord was solubilized with the nonionic detergent Triton X-100 and subsequently purified by affinity chromatography on aminostrychnine-agarose and wheat germ agglutininSepharose. An overall purification of 1950-fold was achieved. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and mercaptoethano1 revealed three glycine receptor-asso...
متن کاملLigand Affinity Chromatography
A simple method for the purification of staphylococcal enterotoxins A (SEA), B (SEB), and C2 (SEC2) from fermentor-grown cultures was developed. The toxins were purified by pseudo-affinity chromatography by using the triazine textile dye "Red A" and gave overall yields of 49% (SEA), 44% (SEB), and 53% (SEC2). The purified toxins were homogeneous when analyzed by sodium dodecyl sulfate-polyacryl...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2018
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.7b03117