Mitotic-inhibiting herbicide response variation in goosegrass (<i>Eleusine indica</i>) with a Leu-136-Phe substitution in α-tubulin

Abstract Dithiopyr and dinitroanilines are preemergence-applied, mitotic-inhibiting herbicides used to control goosegrass [ Eleusine indica (L.) Gaertn.] in turfgrass. A suspected resistant E. population was collected from a golf course putting green evaluated for possible resistance dithiopyr prodiamine. After dose–response evaluation, the α-tubulin gene sequenced known target-site mutations that have been reported confer herbicides. mutation discovered resulted an amino acid substitution at position 136 leucine phenylalanine (Leu-136-Phe). Previous research has indicated Leu-136-Phe does dinitroaniline The level of by regression models I 50 values indicates there is 54.1-, 4.7-, &gt;100-, &gt;100-fold dithiopyr, prodiamine, pendimethalin, oryzalin, respectively, when compared with susceptible based on seedling emergence response 88.4-, 7.8-, biomass reduction response. This first report less prodiamine pendimethalin or oryzalin due associated resistance.