Mitochondrial uncouplers induce proton leak by activating AAC and UCP1
نویسندگان
چکیده
We provide notes on how to use a graphical user interface (GUI), implemented with MATLAB, for aligning imaging datasets of biological tissue. The original was matching two data sets, where one set taken the living preparation and another post-fixation following immunohistochemical processing. This technique is described in detail an accompanying paper (Parrish et al., [1], we also include information about experimental procedures, examples usage GUI.
منابع مشابه
Increased mitochondrial proton leak in skeletal muscle mitochondria of UCP1-deficient mice.
Mice having targeted inactivation of uncoupling protein 1 (UCP1) are cold sensitive but not obese (Enerbäck S, Jacobsson A, Simpson EM, Guerra C, Yamashita H, Harper M-E, and Kozak LP. Nature 387: 90-94, 1997). Recently, we have shown that proton leak in brown adipose tissue (BAT) mitochondria from UCP1-deficient mice is insensitive to guanosine diphosphate (GDP), a well known inhibitor of UCP1...
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Mice deficient in mitochondrial uncoupling protein (UCP) 1 are cold sensitive, despite abundant expression of the homologues, Ucp2 and Ucp3 (S. Enerbäck, A. Jacobsson, E. M. Simpson, C. Guerra, H. Yamashita, M.-E. Harper, and L. P. Kozak. Nature 387: 90-94, 1997). We have analyzed characteristics of mitochondrial proton leak from brown adipose tissue (BAT) of Ucp1-deficient mice and normal cont...
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An energetically significant leak of protons occurs across the mitochondrial inner membranes of eukaryotic cells. This seemingly wasteful proton leak accounts for at least 20% of the standard metabolic rate of a rat. There is evidence that it makes a similar contribution to standard metabolic rate in a lizard. Proton conductance of the mitochondrial inner membrane can be considered as having tw...
متن کاملMitochondrial proton leak and the uncoupling protein 1 homologues.
Mitochondrial proton leak is the largest single contributor to the standard metabolic rate (SMR) of a rat, accounting for about 20% of SMR. Yet the mechanisms by which proton leak occurs are incompletely understood. The available evidence suggests that both phospholipids and proteins in the mitochondrial inner membrane are important determinants of proton conductance. The uncoupling protein 1 h...
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ژورنال
عنوان ژورنال: Biochimica Et Biophysica Acta - Bioenergetics
سال: 2022
ISSN: ['1879-2650', '0005-2728']
DOI: https://doi.org/10.1016/j.bbabio.2022.148719