Mitochondrial Genome of Brugia malayi Microfilariae Isolated From a Clinical Sample

Lymphatic filariasis is a neglected parasitic disease that leading cause of long-term disability. Information obtained from genome sequencing filarial worm can help us identify systems in the are likely to be useful for novel drug design. Brugia ( B .) malayi still only lymphatic-dwelling parasite with nearly complete, fully annotated, and published genome. However, most previous studies were based on FR3 strain B. , which originally was isolated human patient, adapted rodent model, then maintained laboratories more than 60 years. It uncertain whether genetic variation exists, thus, clinical isolates lymphatic dwelling parasites high priority. Here, we report first time complete mitochondrial microfilariae isolate. Complete (mt) blood sample taken Thai subject living Narathiwat Province, an endemic area brugian filariasis, assembled reads by Illumina sequencing. Gene annotation, phylogenetic analysis single nucleotide polymorphism (SNP) deployed. A 13,658-bp mt . microfilaria obtained, it shows 68x coverage. Based gene consists 12 protein-coding, two rRNA, 23 tRNA genes. Phylogenetic using all protein sequences DNA or cytochrome c oxidase subunit I COX1 ) revealed close relationship among three filariae (i.e., timori zoonotic pahangi Wuchereria spp.). The SNPs differentiate those found canine. Furthermore, number, order transcription, direction genes same as comparison could have important implications development new intervention vaccine treat prevent this areas/regions around world.