Mitochondrial DNA replication does not involve DNA polymerase alpha.
نویسندگان
چکیده
منابع مشابه
Mitochondrial DNA Replication Does Not InvolveD NA Polymerase a
Aphidicolin, a known inhibitor of DNA polymerase a, is a potent inhibitor of nuclear DNA synthesis in HeLa cells but has no effect on the replication f mitochondrial DNA. Parallel experiments with mitochondria incubated in ui tm also show no inhibition of DNA synthesis by aphidicolin; however, DNA synthesis in these isolated mitochondria is completely blocked by dideoxycytidine triphosphate, wh...
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The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of replication activity. The addition of purified pol alpha-primase complex isolated f...
متن کاملHuman mitochondrial DNA replication.
Elucidation of the process of DNA replication in mitochondria is in its infancy. For many years, maintenance of the mitochondrial genome was regarded as greatly simplified compared to the nucleus. Mammalian mitochondria were reported to lack all DNA repair systems, to eschew DNA recombination, and to possess but a single DNA polymerase, polymerase γ. Polγ was said to replicate mitochondrial DNA...
متن کاملThe exonuclease activity of DNA polymerase γ is required for ligation during mitochondrial DNA replication
Mitochondrial DNA (mtDNA) polymerase γ (POLγ) harbours a 3'-5' exonuclease proofreading activity. Here we demonstrate that this activity is required for the creation of ligatable ends during mtDNA replication. Exonuclease-deficient POLγ fails to pause on reaching a downstream 5'-end. Instead, the enzyme continues to polymerize into double-stranded DNA, creating an unligatable 5'-flap. Disease-a...
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HOMOLOGY TO REPLICATION PROTEIN A-32 REGULATES DNA REPLICATION IN MAMMALIAN CELLS Darren E. Casteel, Shunhui Zhuang, Ying Zeng, Fred W. Perrino*, Gerry R. Boss, Mehran Goulian, and Renate B. Pilz From the Department of Medicine and Cancer Center of the University of California, San Diego, La Jolla, CA 92093, and *the Department of Biochemistry and Cancer Center of Wake Forest University, Winsto...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)70211-7