Mitochondrial deoxyribonucleic acid from Tetrahymena pyriformis and its kinetic complexity
نویسندگان
چکیده
منابع مشابه
Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants.
1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distributio...
متن کاملMitochondrial and Cytoplasmic Ribosomes from Tetrahymena Pyriformis
Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis have been isolated and studied by the techniques of polyacrylamide gel electrophoresis and electron microscopy used in conjunction. Although the two ribosome types show the same coefficient of sedimentation (80S) in sucrose gradients, they can be distinguished by gel electrophoresis: mitoribosomes migrate in a single band, cons...
متن کاملPreparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.
A method is described that enables a chromatin fraction containing ribosomal DNA (DNA containing sequences coding for rRNA) to be prepared from the macronuclei of growing or stationary cultures of Tetrahymena pyriformis. This material is obtained in yields of between 25 and 75% of the theoretical maximum. The DNA in this fraction was identified as ribosomal DNA on the basis of its density and m...
متن کاملFatty acids of mitochondrial membranes from Tetrahymena pyriformis.
We have examined the fatty acid composition of the mitochondrial membranes in three strains of Tetrahymena pyriformis. All three had similar components and exhibited large amounts of unsaturated fatty acids. The cytoplasmic mutant, CA-10, which has a slower growth rate and unusual membrane morphology, had a slightly higher amount of iso-acids but was otherwise similar to the other strains in fa...
متن کاملSynthesis of Deoxyribonucleic Acid by Micro- and Macronuclei of Tetrahymena Pyriformis
Evidence as to the times of DNA synthesis in micronucleate Tetrahymena pyriformis (mating type II, variety 1) has been obtained by briefly exposing individuals of different ages to tritiated thymidine, returning them to non-radioactive medium, fixing at division, and preparing autoradiographs. A variable length of interphase, ranging from a few minutes to about 2 hours, has been found to preced...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1160811