Mitochondrial d-β-Hydroxybutyrate Dehydrogenase
نویسندگان
چکیده
منابع مشابه
3-hydroxybutyrate dehydrogenase
The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as substrate was > 10% of that with 3-hydroxybutyrate. However, ...
متن کامل3-Hydroxyisobutyrate dehydrogenase, an impurity in commercial 3-hydroxybutyrate dehydrogenase.
The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3-hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as substrate was greater than 10% of that with 3-hydroxybutyrat...
متن کاملRegulation of D-0-Hydroxybutyrate Dehydrogenase in Rat Hepatoma Cell Lines
Quantitation of i>-/3~liy butvratc dehydrogenase (BDH) in normal rat hepatocytes was compared with that in two rat hepatoma cell lines, H4-II-EC3 and RLT-3C. BDH activity in normal rat hepatocyte mito chondria was 321 nmol/min/mg, which was greatly reduced to 10.7 nmol/ min/mg and 1.7 nmol/min/mg in H4-II-EC3 and RLT-3C cell mitochon dria, respectively. The cell growth rate and L-[35Slmet...
متن کاملCrystallization and preliminary X-ray characterization of D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi.
A recombinant form of D-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b = 99.0, c = 110.2 A. The crystals are most likely to contain two tetrameric subunits in the asymmetric unit, with a VM...
متن کاملEffects of inhibitors on mitochondrial D-alpha-hydroxy acid dehydrogenase.
Previous papers (Tubbs & Greville, 1959; Tubbs, 1960a; Tubbs & Greville, 1961) have reported properties of a D-o-hydroxy acid dehydrogenase extracted in soluble form from acetone-dried rabbit-kidney mitochondria. An account of some studies on this enzyme has been presented elsewhere (Tubbs, 1960b), and various effects of metalcomplex-forming agents on the activity of the enzyme have been the ba...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44144-6