Misclassification of PfEH1 and PfEH2 as Epoxide Hydrolases
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Misclassification of PfEH1 and PfEH2 as Epoxide Hydrolases
In their recent article (1), Spillman and colleagues report the two Plasmodium falciparum proteins PfEH1 and PfEH2 to be two novel epoxide hydrolases with an atypical active-site architecture. We strongly believe that this conclusion is wrong for the reasons detailed in the following. Spillman et al. start from the observation that, based on sequence identity, epoxide hydrolases from the / hydr...
متن کاملReply to “Misclassification of PfEH1 and PfEH2 as Epoxide Hydrolases”
In our recent study, we characterized two exported proteins, PfEH1 and PfEH2, of Plasmodium falciparum (1). Overexpression of these proteins resulted in a decrease in epoxide-containing lipids in infected erythrocytes, which we proposed was due to the epoxide hydrolase activities of these / hydrolases. We appreciate the thoughtful comments of Arand and Marowsky (2), who propose an alternative e...
متن کاملExported Epoxide Hydrolases Modulate Erythrocyte Vasoactive Lipids during Plasmodium falciparum Infection
Erythrocytes are reservoirs of important epoxide-containing lipid signaling molecules, including epoxyeicosatrienoic acids (EETs). EETs function as vasodilators and anti-inflammatory modulators in the bloodstream. Bioactive EETs are hydrolyzed to less active diols (dihydroxyeicosatrienoic acids) by epoxide hydrolases (EHs). The malaria parasite Plasmodium falciparum infects host red blood cells...
متن کاملEpoxide hydrolases: biochemistry and molecular biology.
Epoxides are organic three-membered oxygen compounds that arise from oxidative metabolism of endogenous, as well as xenobiotic compounds via chemical and enzymatic oxidation processes, including the cytochrome P450 monooxygenase system. The resultant epoxides are typically unstable in aqueous environments and chemically reactive. In the case of xenobiotics and certain endogenous substances, epo...
متن کاملEnhancement of Soluble Expression and Biochemical Characterization of Two Epoxide Hydrolases from Bacillus
Background: Enantiopure epoxides are important intermediates in the synthesis of high-value chiral chemicals. Epoxide hydrolases have been exploited in biocatalysis for kinetic resolution of racemic epoxides to produce enantiopure epoxides and vicinal diols. It is necessary to obtain sufficient stable epoxide hydrolases with high enantioselectivity to meet the requirements of i...
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ژورنال
عنوان ژورنال: mBio
سال: 2017
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.00004-17