Miracle Fruit Pulp Transcriptomes Identify Genetic Variants in Support of Discovery Research and Breeding

Miracle fruit ( Synsepalum dulcificum ) is the botanical source of miraculin, a natural, noncaloric sweetener. plants have bush-like architecture and produce multiple flushes attractive red berries each year. The consist large seed, opaque pulp, brilliant peel. pulp contains glycoprotein, that binds to tongue’s sweet receptors induces conformational change in response acidic stimuli. Thus, strong sensation imparted absence sugars. miracle plant becoming increasingly popular because its taste-modifying properties, but species lacks many breeding tools common other crops. We report transcriptomes from ‘Sangria’, ‘Vermilion’, ‘Flame’, ‘Cherry’ morphotypes. A consensus transcriptome included 91,856 transcripts. Reads mapping miraculin gene had highest representation individual transcriptomes. Other abundant transcripts primarily Gene Ontology categories representing cellular components, nucleus nucleic acid binding, protein modification. were used design real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) primers for actin, elongation factor 1α, gene. Analysis by qRT-PCR indicated peel tissues abundance transcripts, although such as leaf, root, flower also detectable levels target sequence. Overall, these results will support discovery research eventual this species.