Mini-αA-Crystallin Stifled Melittin-Induced Haemolysis and Lymphocyte Lysis

Abstract Melittin, the most potent pharmacological ingredient of honey bee venom, induces haemolysis, lymphocyte lysis, long-term pain, localised inflammation, and hyperalgesia. In this study, efforts were made to subdue melittin’s ill effects using a chaperone peptide called ‘mini-αA-crystallin’ (MAC) derived from eye lens αA-crystallin. Haemolytic test on human red blood cells, percentage viability, DNA diffusion assay Human peripheral lymphocytes (HPBLs) performed with melittin in presence or absence MAC. Propidium iodide Annexin V-FITC dual staining analyse quantitative levels necrotic apoptotic induction by MAC HPBLs flow cytometer. A computational study find out interactions between was undertaken modelling structure PEP-FOLD server. The result showed that inhibited melittin-induced lysis nucleated (lymphocytes) enucleated (RBC) cells. Flow cytometric analysis revealed substantial increase late cells after treating (4 µg/ml) for 24 h. Treatment at 2:1 molar ratio prevented developing necrosis apoptosis. docking hydrogen, van der Waals, π-π stacking, salt bridges observed complex, confirming strong interaction them. MAC-melittin complex stable during molecular dynamics simulation. These findings may be beneficial medication severe cases honeybee stings.