Millisecond analysis of double stranded DNA with fluorescent intercalator by micro-thermocontrol-device
نویسندگان
چکیده
منابع مشابه
Selective detection of Hg2+ in the microenvironment of double-stranded DNA with an intercalator crown-ether conjugate.
9-[2-(1,4-Dioxa-7,13-dithia-10-azacyclopentadecyl)phenyl]amino-benzo[b]quinolizinium enables the unambiguous fluorimetric and polarimetric detection of Hg(2+) in the close proximity of double-stranded nucleic acids without interfering background signals from the complexes of this compound with Hg(2+) or DNA alone.
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Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequence...
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The synthesis, proof of structure, and the absorption and fluorescence properties of two new unsymmetrical cyanine dyes, thiazole orange dimer (TOTO; 1,1'-(4,4,7,7-tetramethyl-4,7- diazaundecamethylene)-bis-4-[3-methyl-2,3-dihydro-(benzo-1,3-thiaz ole)-2- methylidene]-quinolinium tetraiodide) and oxazole yellow dimer (YOYO; an analogue of TOTO with a benzo-1,3-oxazole in place of the benzo-1,3-...
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Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed to either a poly(dA) or a poly(rA) backbone and, remarkably, free oligo(dT). Th...
متن کاملMiniaturized thermocontrol devices enable analysis of biomolecular behavior on their timescales, second to millisecond.
To establish general-purpose methods and tools for biological experiments on a short time scale is an essential requirement for future research in molecular biology because most of the functions of living organisms at the molecular level take place on a time scale from 1-second to millisecond. Thermal control with on-chip micro-thermodevices is one of the strongest and most useful ways to reali...
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ژورنال
عنوان ژورنال: Talanta
سال: 2009
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2009.04.045