Migration of transfused granulocytes in leukopenic dogs

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Migration of transfused granulocytes in leukopenic dogs.

Although granulocyte transfusion therapy has been shown to be effective in infected granulocytopenic animals and humans, the relative effectiveness of granulocytes (PMN) harvested by continuous flow centrifugation (CFC) or by continuous flow filtration leukapheresis (FL) remains uncertain. Studies in vitro of morphology and granulocyte functions have suggested cells collected by FL may be damag...

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Granulocyte transfusions in leukopenic dogs: in vivo and in vitro function of granulocytes obtained by continuous-flow filtration leukopheresis.

The present studies were carried out to (1) evaluate a leukoadhesive technique for obtaining granulocytes for transfusion, (2) assess the granulocytes by in vitro techniques, and (3) determine the efficacy of granulocyte transfusion in preventing sepsis in leukopenic dogs. Dogs were rendered transiently leukopenic (<500 per cu mm) by intravenous cyclophosphamide, 40 mg/kg. Quantitative and qual...

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Alloimmunization prevents the migration of transfused indium-111-labeled granulocytes to sites of infection.

111In-labeled granulocytes were used to study the effects of histocompatibility factors on the migration of transfused granulocytes to infected sites. Fourteen alloimmunized and 20 nonalloimmunized patients received approximately 10(8) 111In-labeled granulocytes from ABO-compatible, non-HLA-matched donors, and scans were performed over known infected sites. All 14 alloimmunized patients had lym...

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Alloimmunization Prevents the Migration of Transfused Indium - ill - Labeled Granulocytes to Sites of Infection

“In-labeled granulocytes were used to study the effects of histocompatibility factors on the migration of transfused granulocytes to infected sites. Fourteen alloimmunized and 20 nonalloimmunized patients received approximately 108 “In-labeled granulocytes from ABO-compatible. non-HIAmatched donors. and scans were performed over known infected sites. All 14 alloimmunized patients had lymphocyto...

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Granulocyte transfusion therapy of experimental Pseudomonas pneumonia.

Pseudomonas pneumonia was produced in dogs with radiation-induced leukopenia. Treatment of this infection with either gentamicin alone or gentamicin plus daily granulocyte transfusion was compared in a randomized controlled trail. The dogs receiving granulocytes plus gentamicin survived significantly longer than those treated with gentamicin alone (P < 0.05). The Pseudomonas immunotype which wa...

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ژورنال

عنوان ژورنال: Blood

سال: 1977

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v49.3.483.483