منابع مشابه
Microtubule dynamics in fish melanophores
We have studied the dynamics of microtubules in black tetra (Gymnocorymbus ternetzi) melanophores to test the possible correlation of microtubule stability and intracellular particle transport. X-rhodamine-or caged fluorescein-conjugated tubulin were microinjected and visualized by fluorescence digital imaging using a cooled charge coupled device and videomicroscopy. Microtubule dynamics were e...
متن کاملMicrotubule system of isolated fish melanophores as revealed by immunofluorescence microscopy
The microtubule system of melanophores of the angelfish, Pterophyllum scalare, has been studied using antibodies prepared against purified porcine brain tubulin in indirect immunofluorescence microscopy. Melanophores were freed from the surrounding tissue components of isolated scales by mild enzymatic digestion and then allowed to settle on a glass cover slip. In both the dispersed and the agg...
متن کاملMyosin cooperates with microtubule motors during organelle transport in melanophores
Melanophores offer an outstanding system for the study of intracellular motility. These cells aggregate their pigment-filled melanosomes to the cell center or disperse them throughout the cytoplasm in response to hormonal modulation of intracellular cyclic AMP levels in order to effect color changes in lower vertebrates [1]. Previous work from our laboratory demonstrated a role for microtubule-...
متن کاملMultiscale trend analysis of microtubule transport in melanophores.
Microtubule-based transport is critical for trafficking of organelles, organization of endomembranes, and mitosis. The driving force for microtubule-based transport is provided by microtubule motors, which move organelles specifically to the plus or minus ends of the microtubules. Motor proteins of opposite polarities are bound to the surface of the same cargo organelle. Transport of organelles...
متن کاملMicrotubule dynamics.
Microtubules are highly dynamic and switch stochastically between growing and shrinking phases both in vivo and in vitro. This non-equilibrium behavior, known as dynamic instability, is based on the binding and hydrolysis of GTP at the nucleotide exchangeable site (E-site) in β-tubulin. Only dimers that have GTP in their E-site can polymerize (red tubulin subunits), but following polymerization...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1994
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.126.6.1455