Microsporogenesis in faba bean (Vicia faba L.) grown in Mersin, Turkey

Legumes are an alternative to animal foods due their high protein content. Like other legumes, Vicia faba (faba bean) has However, bean breeding with classical methods is challenging inbreeding depression, self-incompatibility, and abortive embryo formation. The inadequacy of the problems in fertilization biology plant limits production new varieties. Therefore, importance using vitro haploidization technique legume increasing. Anther culture a widely preferred tissue for obtaining haploid plants. initial most crucial stage anther identification anthers containing microspores appropriate developmental stage. In study, samples single triple-nucleated were cultured MS nutrient medium 2,4-D (0.5 mg L-1) Kinetin (2.5 L-1). first month culture, embryoid-like structures obtained at heart from mononuclear stage, while trinucleated observed darken did not develop.