Microscopic scan-free surface profiling over extended axial ranges by point-spread-function engineering
نویسندگان
چکیده
منابع مشابه
Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-f...
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ژورنال
عنوان ژورنال: Science Advances
سال: 2020
ISSN: 2375-2548
DOI: 10.1126/sciadv.abc0332