Microscale Adaptation ofIn VitroTranscription/Translation for High-Throughput Screening of Natural Product Extract Libraries
نویسندگان
چکیده
منابع مشابه
High-Throughput Screen of Natural Product Libraries for Hsp90 Inhibitors
Hsp90 has become the target of intensive investigation, as inhibition of its function has the ability to simultaneously incapacitate proteins that function in pathways that represent the six hallmarks of cancer. While a number of Hsp90 inhibitors have made it into clinical trials, a number of short-comings have been noted, such that the search continues for novel Hsp90 inhibitors with superior ...
متن کاملMarine Natural Product Libraries for High-Throughput Screening and Rapid Drug Discovery
There is a need for diverse molecular libraries for phenotype-selective and high-throughput screening. To make marine natural products (MNPs) more amenable to newer screening paradigms and shorten discovery time lines, we have created an MNP library characterized online using MS. To test the potential of the library, we screened a subset of the library in a phenotype-selective screen to identif...
متن کاملNatural product libraries: assembly, maintenance, and screening.
This review discusses successful strategies and potential pitfalls to assembling a natural product-based library suitable for high-throughput screening. Specific extraction methods for plants, microorganisms, and marine invertebrates are detailed, along with methods for generating a fractionated sub-library. The best methods to store, maintain and prepare the library for screening are addressed...
متن کاملHigh-throughput method for the production and analysis of large natural product libraries for drug discovery.
High-throughput methods were applied to the production, analysis, and characterization of libraries of natural products in order to accelerate the drug discovery process for high-throughput screening in the pharmaceutical and biotechnology industries. Library production integrates automated flash chromatography, solid-phase extraction, filtration, and high-throughput parallel four-channel prepa...
متن کاملIntegration of high-content screening and untargeted metabolomics for comprehensive functional annotation of natural product libraries.
Traditional natural products discovery using a combination of live/dead screening followed by iterative bioassay-guided fractionation affords no information about compound structure or mode of action until late in the discovery process. This leads to high rates of rediscovery and low probabilities of finding compounds with unique biological and/or chemical properties. By integrating image-based...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical Biology & Drug Design
سال: 2015
ISSN: 1747-0277
DOI: 10.1111/cbdd.12614