Microsatellite Primers for the Rare SedgeLepidosperma bungalbin(Cyperaceae)
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چکیده
منابع مشابه
Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae)1
PREMISE OF THE STUDY Microsatellite markers were developed for the rare sedge Lepidosperma bungalbin (Cyperaceae) to assess genetic variation and its spatial structuring. METHODS AND RESULTS We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which...
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PREMISE OF THE STUDY Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. METHODS AND RESULTS Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identif...
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Microsatellites abound in most organisms and have proven useful for a range of genetic and genomic studies. Once primers have been created, they can be applied to populations or taxa that have diverged from the source taxon. We use PCR amplification, in a 96-well format, to determine the presence and absence of 46 microsatellite loci in 13 cichlid species. At least one primer set amplified a pr...
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UNLABELLED PREMISE OF THE STUDY Rhizopogon kretzerae and R. salebrosus (Rhizopogonaceae) are ectomycorrhizal fungi symbiotic with pines and the mycoheterotrophic plant Pterospora andromedea (Ericaceae). Microsatellite loci will allow population genetic study of fungal hosts to P. andromedea. • METHODS AND RESULTS Shotgun pyrosequencing of R. kretzerae DNA resulted in primer development of ...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2016
ISSN: 2168-0450
DOI: 10.3732/apps.1600083