MicroRNA 744 induces apoptosis and autophagy in human gastric cancer by targeting heparanase-1

Purpose: To study the possible anti-cancer effects of miR-744 on human gastric cancer cells, and underlying mechanism.
 Methods: The mRNA expression was determined using qRT-PCR, while CCK-8, Annexin V/PI acridine orange assays were used to determine cell viability, apoptosis autophagy, respectively. expressions target proteins qRT-PCR Immunoblotting.
 Results: Significant suppressions observed in tissues lines. Overexpression inhibited proliferation silencing promoted cells. Furthermore, tumor-suppressive effect due induction autophagy. Heparanase-1 (HPSE-1) identified as miR-744. Silencing HSPE-1 significantly proliferation, whereas its overexpression restored growth-inhibitory (p < 0.05).
 Conclusion: These results indicate that exerts a growth cells vitro by targeting HPSE-1. There is need further animal models.