microR-1294-5p inhibits glycolytic metabolism of non-small cell lung cancer cells via targeting TMPRSS11B

Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust growth resist death. MicroRNAs (miRNAs) have been known play pivotal roles in NSCLC development partly through mediating glycolysis. However, only a few miRNAs experimentally confirmed as critical regulators of NSCLC. TCGA datasets were analyzed screen for differentially expressed between normal tissues. The function miR-1294-5p was determined proliferation, uptake, lactate release, Extracellular Acidification Rate (ECAR) assays. target miR- 1294-5p predicted TargetScan miRDB, which further validated flow cytometry analysis, RT-qPCR, western blotting, dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay. In the present study, it found that significantly downregulated miRNA adenocarcinoma (LUAD) squamous carcinoma (LUSC). overexpression inhibited glycolysis, export, ECAR, proliferation cells. Analysis with bioinformatic tools, Western Blotting, RIP assay showed directly bound complementary sites 3’-Untranslated Region (UTR) TMPRSS11B resulted downregulation expression. addition, transfection recombinant rescued functions on findings provided novel insights understanding regulation glycolytic metabolism