Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry, as well considered an endangered species. Problems with traditional propagation methods limit development large-scale commercial production system. The use micropropagation has been investigated this species, and liquid in vitro systems showed potential temporary immersion systems, also called bioreactors (TIB) B. nodosa. This study evaluated efficiency SETIS™ TIBs Explants consisting young shoots tips measuring between 0.3 0.5 cm were collected from 1-yr-old seedlings used shoot multiplication. Four culture evaluated: semi-solid system 160 mm × 100 TP1600 Microbox containing 500 ml medium control, 4 L bioreactor (SETIS™, VERVIT, Belgium) aeration frequencies 2 h, 8 h. For all treatments, duration was min. Results that explants frequency h min returned highest multiplication rates, 4.6 produced per explant compared 2.8 agar-based systems. promoted increased growth rooting, therefore improving survival, facilitating acclimatization vitro-derived plantlets. first demonstrating successful protocol using bioreactors, which could have significant value impact species conservation purposes. Micropropagation achieved SETIS good thus survival ex vitro.