Microplate enzyme-linked immunosorbent assay for bovine leukemia virus antibody.

Microplate enzyme-linked immunosorbent assay for bovine leukemia virus antibody.

A microplate enzyme-linked immunosorbent assay method was developed for the measurement of bovine immunoglobulin G antibody specific to the envelope antigen (glycoprotein 60) of bovine leukemia virus. The test was then performed on 440 serum samples from dairy cows belonging to herds in which bovine leukemia was suspected or which were leukemia free, and the results were compared with those obt...

Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus

The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...

Antibody assay for Japanese encephalitis virus in bovine serum by enzyme-linked immunosorbent assay (ELISA).

adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51su for detection of antibodies to bovine leukemia virus

the objective of this study was to compare an indirect elisa, based on a purified 60 kda envelope glycoprotein (gp51su), with a pourquire indirect elisa for the detection of antibodies to the bovine leukemia virus. for conducting this research, 340 serum samples were collected from two different breeds of cows (sarabi and holestin) in different herds. commercial elisa revealed positive results ...