Microfluidic Western Blotting of Low-Molecular-Mass Proteins
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چکیده
منابع مشابه
Microfluidic Western Blotting of Low-Molecular-Mass Proteins
We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5-116 kDa). The Tris tricine μWestern is completed in an enclosed, straight glass microfluidic channel housing a photopatterned polyacrylamide gel that incorporates a photoactive benzophenone methacrylamide monomer. Upon brief ultravio...
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Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ antibody probing. To validate the microfluidic...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2014
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac5024588