Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns
نویسندگان
چکیده
منابع مشابه
Real-Time PCR for Gene Expression Analysis
After discovery of polymerase chain reaction (PCR) by Dr. Kary Mullis in 1983, several different types of PCR have been invented and continually improved upon over the years. One of them called "Real-time PCR" or “fluorescence based PCR” allows us to quantitate nucleic acids obtained from cells or tissues, to compare the variable states of infection, to detect chromosomal translocations, to gen...
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Mikael Kubista, TATAA Biocenter and MultiD Analyses AB, Sweden, Björn Sjögreen, Center for Applied Scientific Computing, Lawrence Livermore National Laboratory, United States and MultiD Analyses AB, Amin Forootan, MultiD Analyses AB, Radek Sindelka and Jiri Jonák, Laboratory of Gene Expression, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic and José Manuel Andrade, D...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2012
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2012.021