منابع مشابه
MicroED data collection and processing
MicroED, a method at the intersection of X-ray crystallography and electron cryo-microscopy, has rapidly progressed by exploiting advances in both fields and has already been successfully employed to determine the atomic structures of several proteins from sub-micron-sized, three-dimensional crystals. A major limiting factor in X-ray crystallography is the requirement for large and well ordered...
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This paper describes a protocol to protect data collected by mobile agents roaming through a set of potentially malicious hosts. This protocol is based on an original secure cryptographic technique that assures the integrity of a sequence of data segments regardless of the order of each segment in the sequence. The protocol allows each host to update the data it previously submitted in a way th...
متن کاملTaking the measure of MicroED.
It is now possible to routinely determine atomic resolution structures by electron cryo-microscopy (cryoEM), facilitated in part by the method known as micro electron-diffraction (MicroED). Since its initial demonstration in 2013, MicroED has helped determine a variety of protein structures ranging in molecular weight from a few hundred Daltons to several hundred thousand Daltons. Some of these...
متن کاملProtein structure determination by MicroED.
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozy...
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The physical model of geyser oscillations presents a challenge to experts, even under relatively simple and clear laboratory circumstances. Despite complex dynamics a qualitative analysis can be made based on video recording and real-time temperature and pressure measurements. The simple experiment with Geyser model is rich with several phenomena from the field of thermodynamics that are includ...
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ژورنال
عنوان ژورنال: Ultramicroscopy
سال: 2019
ISSN: 0304-3991
DOI: 10.1016/j.ultramic.2019.03.009