Microdetermination of hypoxanthine and xanthine
نویسندگان
چکیده
منابع مشابه
Xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as follows
The enzyme is well-suited to carry out the catalysis of these oxidative reactions, containing as it does four redox-active sites: molybdenum, flavin adenine dinucleotide (FAD) and a pair of iron-sulfur centers of the ferredoxin type (Fe2S2). The purine substrates interact with the enzyme at the molybdenum site of xanthine oxidase and oxygen at the flavin site. The two metabolic reactions above ...
متن کاملThe reduction of cytochrome c by hypoxanthine and xanthine oxidase.
Ball, E. G. (1939a). J. biol. Chem. 128, 51. Ball, E. G. (1939b). Cold Spr. Harb. Sym. quant. Biol. 7, 100. Ball, E. G. (1946). J. gen. Phy8iol. 29, 413. Corran, H. S., Dewan, J. G., Gordon, A. H. & Green, D. E. (1939). Biochem. J. 38, 1694. Dixon, M. (1938). Enzymologia, 5, 198. Dixon, M. & Keilin, D. (1936). Proc. roy. Soc. B, 119, 159. Green, D. E., Knox, W. E. & Stumpf, P. K. (1941). J. bio...
متن کاملA kinetic study of hypoxanthine oxidation by milk xanthine oxidase.
The course of the reaction sequence hypoxanthine----xanthine----uric acid catalysed by xanthine:oxygen oxidoreductase from milk was investigated on the basis of u.v. spectra taken during the course of hypoxanthine and xanthine oxidations. It was found that xanthine accumulated in the reaction mixture when hypoxanthine was used as a substrate. The time course of the concentrations of hypoxanthin...
متن کاملExamination of performance of glassy carbon paste electrode modified with gold nanoparticle and xanthine oxidase for xanthine and hypoxanthine detection.
A composite electrode was prepared by modifying glassy carbon microparticles with gold nanoparticles (Au-nps) and xanthine oxidase enzyme (XOD) for xanthine (X) and hypoxanthine (Hx) detection. After the optimization of the system for X, the biosensor was characterized for X and Hx. A linearity was obtained in the concentration range between 5.00 x 10(-7) and 1.00 x 10(-5) M for X with equation...
متن کاملCerebrospinal fluid concentrations of hypoxanthine, xanthine, uridine and inosine: high concentrations of the ATP metabolite, hypoxanthine, after hypoxia.
CSF obtained for clinical purposes from newborn, children and adults has been analysed by high pressure liquid chromatography for hypoxanthine, xanthine, inosine, uridine and urate. Large rises in hypoxanthine and to a lesser extent xanthine occur for about 24 h after hypoxia. High concentrations were associated with later evidence of brain damage or subsequent death. Changes in CSF could be in...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1939
ISSN: 0306-3283
DOI: 10.1042/bj0330984