Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping
نویسندگان
چکیده
منابع مشابه
Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping
We have mapped sequence-directed nucleosome positioning on genomic DNA molecules using high-throughput sequencing. Chromatins, prepared by reconstitution with either chicken or frog histones, were separately digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase (CAD). Both enzymes preferentially cleave internucleosomal (linker) DNA, although they do so...
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Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the cutting preference of MNase in combination with size selection generates a sequence-dependent bias in the resultin...
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This report relates to the preceding paper by Taniuchi and Anfinsen. Micrococcal nuclease from the strain “Foggi” has been purified from a commercially available source (Worthington). The enzyme was crystallized from an ammonium sulfate solution. The over-all purification was approximately SOO-fold with an over-all yield of about 16%. The enzyme is virtually devoid of acid and alkaline phosphat...
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The micrococcal nuclease cleavage of intact nuclear chromatin from African green monkey cells and of the completely deproteinized sequences was studied by using high-resolution analytical and DNA sequencing gels and secondary restriction enzyme analysis. When deproteinized component alpha DNA was used as substrate, not all phosphodiester bonds in the 172-base-pair repeat units were cleaved with...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2012
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2012.01.043