Microbial transglutaminase induced modification of wheat gliadin based nanoparticles and its impact on their air-water interfacial properties
نویسندگان
چکیده
There is a growing interest in plant protein based nanoparticles (NPs) such as wheat gliadin NPs (GNPs) for stabilizing foams food systems. To increase the range of their applications, it necessary to understand role different types and tailor behavior at air-water (A-W) interfaces. The latter here done by modifying GNPs with microbial transglutaminase (TG). most pronounced solubility loss ?-gliadins (by 36.1%) after TG treatment pH 4.5, measured reversed phase high performance liquid chromatography (RP-HPLC), indicated that this type was more susceptible action than ?-gliadins ?-gliadins, losses which were 11.2 11.4% respectively. lack intramolecular disulfide bonds but not other likely resulted greater accessibility lysine residues thus susceptibility enzyme. Interestingly, notably improved foaming properties 4.5 (and below pI). surface dilatational modulus value treated GNPs, when compared control (53.9 32.7 mN/m, respectively), formation strong viscoelastic A-W interfacial film, presumably due presence catalyzed intermolecular isopeptide bonds. Fractionation stabilized (un-)treated subsequent RP-HPLC analysis foam fractions showed ?-gliadins important interface stability even if affected treatment. Elimination positively charged (at 4.5) conformational changes (both result treatment) may have altered ability adsorb interact proteins thereat. Overall, very films both covalent non-covalent interactions turn led highly stable foams.
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ژورنال
عنوان ژورنال: Food Hydrocolloids
سال: 2022
ISSN: ['1873-7137', '0268-005X']
DOI: https://doi.org/10.1016/j.foodhyd.2021.107471