Microbial Transformation of Cholesterol into Coprostanol. Properties of a 3-Oxo-Delta4-Steroid-5beta-Reductase

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The transformation of cholesterol-3d to coprostanol-d. Location of deuterium in coprostanol.

Recent work from this laboratory has dealt with the biochemical mechanism by which the conversion of cholesterol to coprostanol was accomplished in human subjects and in incubation experiments (1). The transformation was shown to occur by saturation of the 5,6 double bond of cholesterol without the intermediate formation of a ketone at C-3. Where cholesterol-3&4-C’* was used, an appreciable amo...

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microbial transformation of cholesterol by mycobacterium smegmatis

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Hepatocyte-like cells have been observed in the pancreas of the rat. We examined the bile acid biosynthetic function of these cells to determine whether they were real hepatocytes. This study investigated the existence of two liver-specific enzymes involved in bile acid biosynthesis (cholesterol 7alpha-hydroxylase and delta(4)-3-ketosteroid 5beta-reductase) in the hepatocyte-like cells. We coul...

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ژورنال

عنوان ژورنال: European Journal of Biochemistry

سال: 1973

ISSN: 0014-2956,1432-1033

DOI: 10.1111/j.1432-1033.1973.tb02968.x