Microbial production of glucose oxidase.

The obtain optimum production conditions for Glucose Oxidase biosensor using software Qualtek-4

The Glucose Oxidase (Gox) electrochemical sensors were used to detect a little amount of glucose. In this paper, the pure platinum plate (length of 5 cm and a width of 3 mm), the Ag/AgCl electrode and platinum electrode (diameter of 1 mm) were applied as a working electrode (W.E), reference electrode and auxiliary electrode sequential. Factors measured, glucose concentration and pH is Phosphate...

The obtain optimum production conditions for Glucose Oxidase biosensor using software Qualtek-4

The Glucose Oxidase (Gox) electrochemical sensors were used to detect a little amount of glucose. In this paper, the pure platinum plate (length of 5 cm and a width of 3 mm), the Ag/AgCl electrode and platinum electrode (diameter of 1 mm) were applied as a working electrode (W.E), reference electrode and auxiliary electrode sequential. Factors measured, glucose concentration and pH is Phosphate...

Studies on Production of Glucose Oxidase using Immobilised Thermophilic Microorganisms

Production of Rabbit Antibodies against Purified Glucose Oxidase

Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) result...

Glucose oxidase from Aspergillus niger: Production, characterization and immobilization for glucose oxidation

Glucose oxidase (GOx), a flavoenzyme, from Aspergillus niger was produced, purified and immobilized for glucose oxidation. Maximum activities of 0.6 and 0.31U mg fungus dry weight were obtained as intracellular and extracellular respectively. Optimal production (4000 IU/L) was achieved when basal salt medium was supplemented with sucrose (7.5%), peptone (1.5%) phosphorus (0.2%) and MgSO4 (0.2%)...