Microbial Mutagenicity Assay: Ames Test

Ames II Mutagenicity Assay Technical Documentation

Ames II Mutagenicity Assay Technical Documentation

Statistical features of a base-specific Salmonella mutagenicity assay are considered in detail, following up on a previous Ž . report comparing responses of base-specific Salmonella Ames IIe strains with those of traditional tester strains. In addition to using different Salmonella strains, the new procedure also differs in that it is performed as a microwell fluctuation test, as opposed to the...

The Ames Salmonella/microsome mutagenicity assay.

The Ames Salmonella/microsome mutagenicity assay (Salmonella test; Ames test) is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to gene mutations. The test employs several histidine dependent Salmonella strains each carrying different mutations in various genes in the histidine operon. ...

Nonparametric regression analysis of data from the Ames mutagenicity assay.

The Ames assay has received widespread attention from statisticians because of its popularity and importance to risk assessment. However, investigators have yet to routinely apply modern regression methods that have been available for more than a decade. We study yet another approach, the application of nonparametric regression techniques, not as the ultimate solution but rather as a framework ...

Development of a new bioluminescent mutagenicity assay based on the Ames test.

A newly developed rapid mutagenicity assay based on the adenosine triphosphate (ATP)-bioluminescence technique and the Ames test is described. Salmonella typhimurium strains TA98 and TA100 were exposed in an appropriate liquid medium to the direct mutagens 4-nitroquinoline-N-oxide and methyl methanesulphonate, respectively, and to the indirect mutagen 2-aminoanthracene. Both auxotrophic and pro...