منابع مشابه
Microbial factories under control: auto-regulatory control through engineered stress-induced feedback.
Severely stressed, with their resources depleted, and their cellular machinery working beyond capacity, the host cells that are used for heterologous protein production have no option but to activate their stress response pathways in order to mitigate the accumulating effects of expressing a foreign, possibly toxic, protein at vast quantities. The result is lower protein yield and quality, with...
متن کاملMicrobial Cell Factories
In contrast to bioreactors the metabolites within the microbial cells are converted in an impure atmosphere, yet the productivity seems to be well regulated and not affected by changes in operation variables. These features are attributed to integral metabolic network within the microorganism. With the advent of neo-integrative proteomic approaches the understanding of integration of metabolic ...
متن کاملMicrobial Cell Factories
Background: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. The 18S rRNA and SUC2 mRNA of Saccharomyces cerevisiae...
متن کاملMicrobial Cell Factories
This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below). Articles in Microbial Cell Factories are listed in PubMed and archived at PubMed Central. For information about publishing your research in Microbial Cell Factories or any BioMed Central journal, go to http://www.microbialcellf...
متن کاملMicrobial Cell Factories
Background: Industrial fermentation typically uses complex nitrogen substrates which consist of mixture of amino acids. The uptake of amino acids is known to be mediated by several amino acid transporters with certain preferences. However, models to predict this preferential uptake are not available. We present the stoichiometry for the utilization of amino acids as a sole carbon and nitrogen s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioengineered
سال: 2013
ISSN: 2165-5979,2165-5987
DOI: 10.4161/bioe.21935