Microarrays: Indirect CyDye Labeling
نویسندگان
چکیده
منابع مشابه
Indirect measurements of differential gene expression with cDNA microarrays.
The use of universal RNA reference sets is an increasingly common approach to molecular classification studies with cDNA microarrays. Here we evaluated the reliability of indirect measurements of fluorescence ratios with a common RNA reference as a means of identifying differentially expressed genes. Comparisons of direct and indirect measures of differential gene expression showed a strong ove...
متن کاملDetecting potential labeling errors in microarrays by data perturbation
MOTIVATION Classification is widely used in medical applications. However, the quality of the classifier depends critically on the accurate labeling of the training data. But for many medical applications, labeling a sample or grading a biopsy can be subjective. Existing studies confirm this phenomenon and show that even a very small number of mislabeled samples could deeply degrade the perform...
متن کاملIdentification of crystallin modifications in the human lens cortex and nucleus using laser capture microdissection and CyDye labeling
PURPOSE With aging, lens crystallins undergo post-translational modifications (PTMs) and these modifications are believed to play a major role in age-related cataract development. The purpose of the present study was to determine the protein profiles of crystallins and their PTMs in the cortical and nuclear regions within an aging human lens to gain a better understanding about changes in cryst...
متن کاملStarting from Scratch in Semantic Role Labeling: Early Indirect Supervision
A fundamental step in sentence comprehension involves assigning semantic roles to sentence constituents. To accomplish this, the listener must parse the sentence, find constituents that are candidate arguments, and assign semantic roles to those constituents. Where do children learning their first languages begin in solving this problem? To experiment with different representations that childre...
متن کاملEvaluation and optimization of procedures for target labeling and hybridization of cDNA microarrays.
PURPOSE To evaluate and optimize methods of target labeling and microarray hybridization using eye gene microarrays. Standardized protocols that consistently produce low background and high intensity hybridization with small amounts of starting RNA are needed to extract differentially expressed genes from a pool of thousands of unaltered genes. METHODS Two identical aliquots of RNA from P19 c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113228