Micro-Organisms and their Investigation1

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Acetylation of peptides inhibits their degradation by rumen micro-organisms.

Proteins and peptides were acetylated using acetic anhydride in order to block their N-terminal amino groups and thereby to prevent their hydrolysis by rumen microbial aminopeptidases. The effects of acetylation on peptide breakdown and ammonia production were determined by incubating unmodified and acetylated substrates with sheep rumen micro-organisms in vitro. Ammonia production from casein ...

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Acetylation of peptides inhibits their degradation by rumen micro-organisms

Proteins and peptides were acetylated using acetic anhydride in order to block their N-terminal amino groups and thereby to prevent their hydrolysis by rumen microbial aminopeptidases. The effects of acetylation on peptide breakdown and ammonia production were determined by incubating unmodified and acetylated substrates with sheep rumen micro-organisms in vitro. Ammonia production from casein ...

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ژورنال

عنوان ژورنال: Nature

سال: 1893

ISSN: 0028-0836,1476-4687

DOI: 10.1038/047446a0