Micro-Cell Chamber and Washed Human Spermatozoa

Micro-cell chamber and washed human spermatozoa.

With the development of the Makler counting chamber, the reproducibility and accuracy of computer-assisted semen analysis and of manual determination of sperm concentration and sperm motility were significantly enhanced beyond what is possible with other methods, such as the hemocytometer, the Horwell chamber, the Petroff-Hausser chamber, and Chartpak slides. The use of Micro-Cell, a disposable...

The Detection of Spermatozoa on Washed Forensic Exhibits

P-150: Effect of Vitrification on High Magnification Morphology and Fertility Potential of Human Spermatozoa Cell

Background: Many sperm characteristics including, motility and morphology are important for normal spermatozoa- oocyte intraction. These features may be altered during vitrification. Motile sperm organelle morphology examination (MSOME) is an unstained real time high magnification technique that analysis viable sperm morphology instantly. This study aimed to investigate the influence of vitrifi...

A micro-perfusion chamber for single-cell fluorescence measurements.

A versatile closed micro-perfusion chamber designed for single-cell fluorescence measurements under maximum microscopic magnification is described. Glass coverslips with adherent cells can be attached to the top or bottom of the chamber, depending on whether an inverted or an upright microscope is used. Eight conical holes drilled in the side of the chamber serve for the insertion of plugs with...

Washed cell salvage in surgical patients

BACKGROUND Cell salvage is commonly used as part of a blood conservation strategy. However concerns among clinicians exist about the efficacy of transfusion of washed cell salvage. METHODS We performed a meta-analysis of randomized controlled trials in which patients, scheduled for all types of surgery, were randomized to washed cell salvage or to a control group with no cell salvage. Data we...