Micro-assay method for lactate dehydrogenase activity in dental plaque.
نویسندگان
چکیده
منابع مشابه
Development of a Plaque Reduction Assay as an Antiphage Activity Evaluation Method
Background: Antiviral screening of newly isolated or synthesized compounds is an important matter which requires a reliable antiviral test. In order to address this issue, the development of a rapid antiphage test has been conducted. To achieve this goal, the antiphage activity of three antiviral drugs (Acyclovir, Lamivudine and Trifluridin) against phage CP51 which infects Bacillus cereus (ATC...
متن کاملLactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination.
The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentratio...
متن کاملEstimation of Lactate Dehydrogenase Activity in cerebrospinal fluid in different types of meningitis
Objective: Meningitis is life-threatening condition and examination of the Cerebrospinal fluid (CSF) may not give a precise diagnosis and prognosis of different types of meningitis. Bacterial meningitis is still a very common problem especially in many developing countries. The aims of study are to estimate the importance and significance of Lactate Dehydrogenase (LDH), protein...
متن کاملMethod variation in lactate dehydrogenase isoenzyme determination.
Differences between methods for determining lactate dehydrogenase isoenzymes are illustrated, which can account for discrepant results. They should be taken into consideration in the interpretation of test results for the diagnosis of myocardial infarction.
متن کاملAn automated colorimetric (tetrazolium salt) assay for serum lactate dehydrogenase.
The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to full automation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier. Estimation requires only 5 mm. of incubation, and assays may be run at th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Nihon University School of Dentistry
سال: 1986
ISSN: 1884-2984,0029-0432
DOI: 10.2334/josnusd1959.28.188