Methylation Analysis of Formalin-Fixed, Paraffin-Embedded Sections Using a Nontoxic DNA Extraction Protocol
نویسندگان
چکیده
منابع مشابه
Methylation analysis of formalin-fixed, paraffin-embedded sections using a nontoxic DNA extraction protocol.
DNA methylation is an epigenetic mechanism responsible for gene silencing (4). The methylation status of a gene can be assessed by a variety of methodologies (1,7). These commonly involve sodium bisulfite treatment of genomic DNA that converts unmethylated, but not methylated, cytosine residues to uracils (2), followed by PCR amplification. We have adapted the basic bisulfite modification proto...
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Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...
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Formalin-fixed paraffin-embedded tissues (FFPET) represent the largest source of archival biological material available for genomic studies. In this work we present an advanced protocol for extraction of high quality DNA from FFPET that can be applied in several molecular studies. Although cat mammary tumours (CMT) are the third most frequent tumour in cats the recovery of significant number of...
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BACKGROUND DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60-80%. AIMS To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction. METHOD Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction ste...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2001
ISSN: 0736-6205,1940-9818
DOI: 10.2144/01316md02