Methyl groups as NMR probes for biomolecular interactions
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چکیده
منابع مشابه
Methyl groups as probes for proteins and complexes in in-cell NMR experiments.
Studying protein components of large intracellular complexes by in-cell NMR has so far been impossible because the backbone resonances are unobservable due to their slow tumbling rates. We describe a methodology that overcomes this difficulty through selective labeling of methyl groups, which possess more favorable relaxation behavior. Comparison of different in-cell labeling schemes with three...
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11 Stevens, B., Spectrochim. Acta, 18, 439 (1962). 12Ferguson, J., J. Chem. Phys., to be published. 13 Abbreviations for the synthetic polynucleotides and their hydrogen-bonded states are those of Inman, R. B., and R. L. Baldwin, J. Mol. Biol., 5, 172 (1962). 14 Davidson, N., J. Widholm, U. S. Nandi, R. Jensen, B. M. Olivera, and J. C. Wang, these PROCEEDINGS, 53, 111 (1965). 11 Chamberlin, M. ...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2015
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2015.08.010